Reaumuria soongorica (Pall.) Maxim., a perennial semi-shrub, is widely found in semi-arid areas in northwestern China. It is a resurrection plant that can tolerate prolonged protoplast desiccation conditions and revive upon rehydration. The present study aimed to investigate the salt tolerance mechanisms of calluses induced from R. soongorica. The growth and organic osmolytes under salt stress were examined. Results were summarized as follows. The relative growth rate of R. soongorica callus reached the maximum in 14 days presence of 100 mM NaCl and it was inhibited with further increasing of NaCl concentrations. The callus had higher relative growth rate in low salt concentrations than in high NaCl concentrations or no NaCl, which indicated that R. soongorica callus need salt in the progress of R. soongorica growing. Examination of the osmotic substances under salt stress showed accumulation of proline, glycine betaine and trehalose with increasing of NaCl concentrations, and the strong osmotic adjustment ability was a important factor in the salt resistant mechanism. The results indicated that the calluses of R. soongorica retained similar to other halophytes in response to salt stress.

Download Here