Soil methane production rate from marsh meadow in permafrost regions on the Tibetan Plateau at various temperatures were determined using an anaerobic incubation technique. The results show that there is a significant exponential correlation between methane production rate and temperature and the methane production rate averaged over all soil layers and the initial methane production rate have pronounced comparability (n=4, p<0.05), increasing with temperature. The methane production rate at 0 ℃, 5 ℃, 18 ℃ is 5.7, 171.4 and 3 257 time more than that at -18 ℃. At 0 ℃, 5 ℃, 18 ℃, the vertical profile of methane production rates has “U” shape, with the higher rate at the ranges of 0~20 cm and 20~80 cm, and the lowest rate at the range of 20~80 cm. But at -18 ℃ there is no obvious changing tendency. The average methane production rates of various depths significantly correlate with incubation time at the incubation temperatures of -18 ℃, 0 ℃, 5 ℃, 18 ℃ (n=9, R²=0.55; n=9, R²=0.99; n=9, R²=0.96; n=9, R²=0.96). However, the ranges of the average methane production rates of various soil layers have obvious difference for all incubation temperatures with incubation time of 1~72 hour, for example, 0.005~0.008, 0.93~1.49 and 16.4~30.7 ng·g^-1.h^-1 at -18 ℃, 5 ℃, 18 ℃, with the variation rate of 160%, 160% and 187%. At 0 ℃ the range of methane production rate was 0.02~0.06 ng·g^-1.h^-1, equivalent to a variation rate of 300%, which is the maximum. It is found that the methane production rate has no significant correlation with soil organic carbon, but the methane production rate of various soil layers has significant correlation with soil pH value at the incubation temperature of 0 ℃, 5 ℃, 18 ℃. At the same time, it is revealed that the methane production rate has significant correlation with soil mechanical composition.